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KMID : 0644120100230030078
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Journal of Oriental Gynecology
2010 Volume.23 No. 3 p.78 ~ p.90
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Effects of Clematidis Radix Extract on Osteoclastogenesis and Gene Expression in RANKL-induced RAW 264.7 Cell
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Song Young-Hoon
Yoo Jeong-Eun
Lim Hyun-Jung
Yoo Dong-Youl
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Abstract
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Purpose: This study was performed to evaluate the effect of Clematidis Radix extract(CB) on osteoclast differentiation and gene expression. The osteocastogenesis and gene expression were determined in RANKL-induced RAW 264.7 cell.
Methods: RANKL-induced RAW 264.7 cell with Clematidis Radix extract was stained by TRAP which is expressive marker of osteoclast. The gene expression of RANK, , IL-6, iNOS and Cathepsin, those are factors related to bone resorption, was estimated by using Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Results: Clematidis Radix extract decreased the number of TRAP-positive multi nuclei cell, and decreased the gene expression of RANK, , IL-6, iNOS and Cathepsin K in RANKL-induced RAW 264.7 cell.
Conclusion: It is concluded that Clematidis Radix extract might decrease the bone resorption resulted from decrease of osteoclast differentiation and it’s related gene expression.
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KEYWORD
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RANKL-induced RAW 264.7 Cell, Clematidis Radix, Osteoporosis
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